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Revista de la Facultad de Medicina Humana

English Title

Effect of the aqueous solution of "mashua" on the reproductive capacity of male mus musculus and its implication in preimplantation embryonic development. Preclinical test

Keywords

Mashua, Tropaeolum tuberosum, dominancia fetal, desarrollo pre-implantacional

English Keywords

Mashua, Tropaeolum tuberosum, fetal dominance, pre-implantacional development

Abstract

La "mashua", Tropaeolum tuberosum, es una especie vegetal nativa del Perú y un recurso de gran valor nutricional. Además, son varias las investigaciones realizadas en la última década en las cuales se ha comprobado sus efectos sobre la fertilidad de mamíferos. Objetivo: evaluar el efecto del extracto acuoso de T. tuberosum en el desarrollo de los embriones preimplantacionales de Mus musculus. Métodos; Se administró el extracto acuoso ad libitum, en una concentración de 50 g/Kg de peso corporal a tres grupos de ratones (n=32 – 8 por grupo caso y 7 control) durante 8, 16 y 35 días; el grupo control tomo solo agua. Cada grupo de machos se cruzaron con hembras de edad fértil, la presencia de tapón vaginal nos indicaba el día 0 de desarrollo embrionario. A las 72 h se perfusionaron los cuernos uterinos y se evaluó el grado de desarrollo, condición y morfología embrionarias. Resultados: Se encontraron diferencias entre los grupos control y tratamiento. Discusión: Nuestros resultados demuestran que el extracto acuoso de T. tuberosum altera la calidad del espermatozoide de ratón afectando la capacidad del embrión para desarrollarse normalmente hasta el estadio de blastocisto.

English Abstract

The "mashua", Tropaeolum tuberosum Ruiz & Pavón, 1802 is a plant species native from Peru and a resource of great nutritional value. In addition, there are several investigations carried out in the last decade in which its effects on mammal fertility have been verified. In this work, we evaluate the effect of the aqueous extract of T. tuberosum on the development of the preimplantation embryos of Mus musculus. The aqueous extract was administered ad libitum, in a concentration of 50 g / Kg of body weight, to three groups of mice (n = 24) during 8, 16 and 35 days; the control group drank only tap water. Each group of males were crossed with females of fertile age, the presence of a vaginal plug indicated the 0th day of embryonic development. At 72 h the uterine horns were perfused and the stage of development, condition and embryonic morphology were evaluated. Differences were found between the control and treatment groups. Our results demonstrate that the aqueous extract of T. tuberosum alters the quality of the mouse sperm, affecting the ability of the embryo to develop normally to the blastocyst stage.

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